In vivo imaging of extracellular pH using1H MRSI

2D luminescence imaging of pH in vivo.

Luminescence imaging of biological parameters is an emerging field in biomedical sciences. Tools to study 2D pH distribution are needed to gain new insights into complex disease processes, such as wound healing and tumor metabolism. In recent years, luminescence-based methods for pH measurement have been developed. However, for in vivo applications, especially for studies on humans, biocompatib...

In Vivo pH Imaging with Tc-pHLIP

Purpose: A novel molecular imaging agent has been developed recently, which stains tissues of low extracellular pH [pH (low) insertion peptide, pHLIP]. A pH-dependent process of peptide folding and insertion into cell membranes has been found in vitro. Targeting of acidic solid tumours has been demonstrated in vivo using fluorescence and PET labels. Here, we present proof of feasibility studies...

ERETIC based in vivo H MRSI quantification

Introduction Quantification of metabolite concentrations from spectroscopic images has been performed by a variety of approaches: Absolute quantification based on internal water referencing [1] is the current method of choice. It accounts for B1 variations across the field of view but imposes problems when assessing diseases that lead to alteration of tissue water concentration [2] and relaxati...

Quantitative In Vivo Imaging of Breast Tumor Extracellular Matrix

Mapping extracellular pH in rat brain gliomas in vivo by 1H magnetic resonance spectroscopic imaging: comparison with maps of metabolites.

The value of extracellular pH (pH(e)) in tumors is an important factor in prognosisand choice of therapy. We demonstrate here that pH(e) can be mappedin vivo in a rat brain glioma by (1)H magnetic resonance spectroscopic imaging (SI) of the pH buffer (+/-)2-imidazole-1-yl-3-ethoxycarbonylpropionic acid (IEPA). (1)H SI also allowed us to map metabolites, and, to better understand the determinant...